Enraizamiento de ejes de Mora (Rubus adenotrichus) en medio líquido y su efecto en la aclimatación

The objective of this research was to evaluate the effect of three concentrations of Indol Butyric Acid (IBA) on the in vitro rooting of the “Thornless Wine” blackberry in the Temporary Immersion System and to determine its survival to acclimatization. The liquid cultivation medium consisted of MS salts at 50%, 3% sucrose, pH 5.7. The IBA concentrations of 0.125 (T1), 0.250 (T2) and 0.500 (T3=control) mg/L were also added. In each temporary immersion container, 200 ml of medium and 15 explants were placed. Five minutes immersion time was programmed every 12 hours. The treatments were incubated at 20ºC and 16 hours of light for 8 weeks. ANOVA and Tukey tests were applied to evaluate the length of the stem, the length of the roots, and the number of internodes. The vitroplants were acclimatized in a substrate of vegetable charcoal, and rice peels (3:1:1); and survival was evaluated after a month. The T1 registered the tallest stem (6.49 cm) and was statistically different to T2 and T3. The T3 showed the longest roots (4,73 cm) and was statistically similar to T1 and T2. Treatments showed no statistical difference in the number of internodes (8 average). T2 showed 78.6% of plant survival at month, T3 57.9%, and T1 43.3%. It was concluded that plants from T2 (0.250 mg/L IBA), which showed an average length of about 6 cm stem and root average length of about 4cm, showed the highest survival during the acclimatization stage. This research was carried out in the Costa Rica Institute of Technology during the year 2008.