CULTIVO DE TEJIDOS EN Ficus carica CON MINIESTACAS

This investigation took place at the Biotechnology Research Ce nter of the Costa Ri ca Institute of Technology between the years 2006 and 2008. Its main objective was to implement the in vitro production of figs. For the in-vitro process, 3- centimeter long ministalks taken from vegetatively propagated material were used. These stalks were washed and immersed in a 6 g/l benomil soluti­on (50 % a.i), 6 g/l gentamicin sulfate (2 % a.i) and oxitetracycline hydrochloride (6 % a.i), a­nd 3.5 g/l dimethyldithiocarbamate (76 % a.i) for 90 minutes. Then, a second disinfection took place with Ca lcium Hip oclorite at 3,5 % p/v for 10 minutes. The stalks were inoculated in a complete M&S (1962) with 1 mg/l of Ascorbic Acid and 175 mg/l of Ciprofloxacin. During the multiplication stage, the larger shoots were separated and inoculated with three different treatments, using as the basic medium a complete M&S (1962), supplemented with 1.0; 0.5, and 0 mg/l of BAP. For the rooting stage, two different treatments were implemented- a complete M&S (1962) with 0.5 and 0 mg/l of IBA. 31.67 % of survival and asepsis of the material during the introductory stage and an average of three shoots per explant were achieved after a month of incubation. The highest average of shoots per explant was obtained with the 1.0 mg/l of BAP treatment, and the highest percentage of average roots was achieved with the treatment without a regulator. During the acclimatation stage, we reached 100 % survival.