Caracterización molecular de dos accesiones de Cydonia oblonga

Luis Alvarado-Marchena; Dora Flores-Mora; Randall Chacón-Cerdas; Alexander Schmidt-Durán; Carlos Alvarado-Ulloa

Caracterización molecular de dos accesiones de Cydonia oblonga

Luis Alvarado-Marchena, Dora Flores-Mora, Randall Chacón-Cerdas, Alexander Schmidt-Durán, Carlos Alvarado-Ulloa

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Resumen

The purpose of this research was to evaluate the plastid trnH-psbA spacer and the matK coding sequence as possible markers for the molecular characterization in two accessions of quince ( C. oblonga ), one from Argentina and the other one from Costa Rica. The study was carried out at Center for Biotechnology Research (CIB in Spanish) of the Technological Institute of Costa Rica (ITCR, in Spanish) during 2014. Amplified products were analyzed using MEGA v6.0 software to calculate genetic distances using the Tamura-Nei model.The results showed that the matK sequence presented the highest percentage of variable sites and the highest intraspecific distance, suggesting the presence of polymorphisms between the two accessions. On the other hand, the trnH-psbA spacer for quince had a low ability to discriminate because, at intraspecific level, this locus showed less differences.

Idioma original	Indefinido/desconocido
Publicación	Agronomía Mesoamericana
Estado	Publicada - 2015

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https://www.redalyc.org/articulo.oa?id=43738993016

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title = "Caracterizaci{\'o}n molecular de dos accesiones de Cydonia oblonga",

abstract = "The purpose of this research was to evaluate the plastid trnH-psbA spacer and the matK coding sequence as possible markers for the molecular characterization in two accessions of quince ( C. oblonga ), one from Argentina and the other one from Costa Rica. The study was carried out at Center for Biotechnology Research (CIB in Spanish) of the Technological Institute of Costa Rica (ITCR, in Spanish) during 2014. Amplified products were analyzed using MEGA v6.0 software to calculate genetic distances using the Tamura-Nei model.The results showed that the matK sequence presented the highest percentage of variable sites and the highest intraspecific distance, suggesting the presence of polymorphisms between the two accessions. On the other hand, the trnH-psbA spacer for quince had a low ability to discriminate because, at intraspecific level, this locus showed less differences.",

author = "Luis Alvarado-Marchena and Dora Flores-Mora and Randall Chac{\'o}n-Cerdas and Alexander Schmidt-Dur{\'a}n and Carlos Alvarado-Ulloa",

year = "2015",

language = "Indefinido/desconocido",

journal = "Agronom{\'i}a Mesoamericana",

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TY - JOUR

T1 - Caracterización molecular de dos accesiones de Cydonia oblonga

AU - Alvarado-Marchena, Luis

AU - Flores-Mora, Dora

AU - Chacón-Cerdas, Randall

AU - Schmidt-Durán, Alexander

AU - Alvarado-Ulloa, Carlos

PY - 2015

Y1 - 2015

N2 - The purpose of this research was to evaluate the plastid trnH-psbA spacer and the matK coding sequence as possible markers for the molecular characterization in two accessions of quince ( C. oblonga ), one from Argentina and the other one from Costa Rica. The study was carried out at Center for Biotechnology Research (CIB in Spanish) of the Technological Institute of Costa Rica (ITCR, in Spanish) during 2014. Amplified products were analyzed using MEGA v6.0 software to calculate genetic distances using the Tamura-Nei model.The results showed that the matK sequence presented the highest percentage of variable sites and the highest intraspecific distance, suggesting the presence of polymorphisms between the two accessions. On the other hand, the trnH-psbA spacer for quince had a low ability to discriminate because, at intraspecific level, this locus showed less differences.

AB - The purpose of this research was to evaluate the plastid trnH-psbA spacer and the matK coding sequence as possible markers for the molecular characterization in two accessions of quince ( C. oblonga ), one from Argentina and the other one from Costa Rica. The study was carried out at Center for Biotechnology Research (CIB in Spanish) of the Technological Institute of Costa Rica (ITCR, in Spanish) during 2014. Amplified products were analyzed using MEGA v6.0 software to calculate genetic distances using the Tamura-Nei model.The results showed that the matK sequence presented the highest percentage of variable sites and the highest intraspecific distance, suggesting the presence of polymorphisms between the two accessions. On the other hand, the trnH-psbA spacer for quince had a low ability to discriminate because, at intraspecific level, this locus showed less differences.

UR - https://www.redalyc.org/articulo.oa?id=43738993016

M3 - Artículo

JO - Agronomía Mesoamericana

JF - Agronomía Mesoamericana

ER -