TY - JOUR
T1 - Biometría testicular y características seminales en felinos neotropicales (Carnivora: Felidae) sometidos a cateterismo uretral
AU - Madrigal-Valverde, Mónica
AU - Bittencourt, Rodrigo F.
AU - Filho, Antonio de Lisboa Ribeiro
AU - Lents, Maicon P.
AU - de Azevedo, Milena C.
AU - Barreto, Renata O.
AU - Teixeira, Nayara A.
AU - Mattos, Paulo M.S.
AU - Curvelo, Vitor P.
AU - Gomes, Marta C.
AU - Dantas, Vínicius
AU - de Araujo, Gediendson Ribeiro
AU - Valverde-Abarca, Anthony
N1 - Publisher Copyright:
© 2019, Universidad de Costa Rica. All rights reserved.
PY - 2019/9
Y1 - 2019/9
N2 - Introduction: The urethral catheterization associated with the application of alfa-2 adrenergic agonist drug is a recent development and application technique for the collection of feline semen, with advantages over electro-ejaculation harvesting and epididymal harvesting. Objetives: Seminal collection in neotropical felines using the urethral catheterization technique. Determine the testicular and seminal parameters of samples collected by the urethral cataterization technique. Methods: The technique has been tested on Panthera onca and others small cats. In this study, individuals of Panthera onca, Puma yagouaroundi and Leopardus wiedii were used in captivity at Getulio Vargas Zoobotanical Park, Salvador, Bahia, Brazil. Medetomidine hydrochloride (0.1 mg/kg) and ketamine (5.0 mg/kg) were used as sedatives, the semen was collected by urethral catheterization. In the process were checked the testicular biometry was performed with percutaner and ultrasonography technique. The fresh samples were evaluated for conventional microscopy in the parameters of volume, sperm vigor, total motility, progressive motility, structural and functional integrity of the plasmatic membrane and sperm morphology. The samples were subjected to a freeze-thaw process using three different extenders. The thawed samples were tested using conventional microscopy, automated system analysis and flow cytometry. Results: In this study, the urethral harvest technique was successfully used in the three species. In the literature used don’t have report about seminal parameters in P. yagouaroundi and urethral seminal collection in L. wiedii. The data presented describe the spermatic morphocinetics of the included species in a pioneer study. There were no differences (P > 0.05) in the seminal quality evaluating the extender, nor differences in the integrity of pre and post-freeze membranes of the samples. Conclusion: The urethral catheterization associated with the application of alfa-2 adrenergic agonist drug it’s an efficient technique for semen collection in neotropical cats. The establishment of a potential association of physical characteristics with seminal quality is extremely important information on the applicability of reproductive traits in assisted reproduction programs.
AB - Introduction: The urethral catheterization associated with the application of alfa-2 adrenergic agonist drug is a recent development and application technique for the collection of feline semen, with advantages over electro-ejaculation harvesting and epididymal harvesting. Objetives: Seminal collection in neotropical felines using the urethral catheterization technique. Determine the testicular and seminal parameters of samples collected by the urethral cataterization technique. Methods: The technique has been tested on Panthera onca and others small cats. In this study, individuals of Panthera onca, Puma yagouaroundi and Leopardus wiedii were used in captivity at Getulio Vargas Zoobotanical Park, Salvador, Bahia, Brazil. Medetomidine hydrochloride (0.1 mg/kg) and ketamine (5.0 mg/kg) were used as sedatives, the semen was collected by urethral catheterization. In the process were checked the testicular biometry was performed with percutaner and ultrasonography technique. The fresh samples were evaluated for conventional microscopy in the parameters of volume, sperm vigor, total motility, progressive motility, structural and functional integrity of the plasmatic membrane and sperm morphology. The samples were subjected to a freeze-thaw process using three different extenders. The thawed samples were tested using conventional microscopy, automated system analysis and flow cytometry. Results: In this study, the urethral harvest technique was successfully used in the three species. In the literature used don’t have report about seminal parameters in P. yagouaroundi and urethral seminal collection in L. wiedii. The data presented describe the spermatic morphocinetics of the included species in a pioneer study. There were no differences (P > 0.05) in the seminal quality evaluating the extender, nor differences in the integrity of pre and post-freeze membranes of the samples. Conclusion: The urethral catheterization associated with the application of alfa-2 adrenergic agonist drug it’s an efficient technique for semen collection in neotropical cats. The establishment of a potential association of physical characteristics with seminal quality is extremely important information on the applicability of reproductive traits in assisted reproduction programs.
KW - CASA
KW - Flow citometry
KW - Leopardus wiedii
KW - Panthera onca
KW - Puma yagouaroundi
KW - Semen colection
UR - http://www.scopus.com/inward/record.url?scp=85078044273&partnerID=8YFLogxK
U2 - 10.15517/rbt.v67i4.36224
DO - 10.15517/rbt.v67i4.36224
M3 - Artículo
AN - SCOPUS:85078044273
SN - 0034-7744
VL - 67
SP - 975
EP - 988
JO - Revista de Biologia Tropical
JF - Revista de Biologia Tropical
IS - 4
ER -