TY - JOUR
T1 - Evaluation of ImageJ software in ultrasonic image analysis
T2 - Follicular and luteal morphological characteristics of cattle
AU - Brito, Lindomar Sousa
AU - da Silva Cavalcante, Ana Karina
AU - Rodrigues, Alexandra Soares
AU - Ferraz, Priscila Assis
AU - Bittencourt, Rodrigo Freitas
AU - Maggitti Junior, Luiz Di Paolo
AU - Vasconcelos, Isabela Costa
AU - Carôso, Bia Santos Souza
AU - Lents, Maicon Pereira
AU - Loiola, Marcus Vinicius Galvão
AU - Madrigal-Valverde, Mónica
AU - Bastos, Morgana Cardoso Brasileiro Borges
AU - de Brito, Osvaldo Santos
AU - de Lisboa Ribeiro Filho, Antônio
N1 - Publisher Copyright:
© 2021 Elsevier B.V.
PY - 2022/1
Y1 - 2022/1
N2 - This study was conducted to compare the effectiveness of two methodologies in evaluating B- and Doppler-mode ultrasonic images: analysis using ultrasonic software and utilizing a computer with ImageJ software. To determine if ImageJ software utilization is an efficacious alternative to ultrasonic software device- analysis, there were comparisons of values when using the two methods for morphological and vascular characteristics of follicular dynamics and luteal function in 18 crossbred cattle. From day 8 of an ovarian dynamics synchronization treatment regimen period until the time of ovulation (Day 10), B-mode and power-flow ultrasonic cineloops were obtained every 12 h to assess follicular diameter, wall area, and wall blood perfusion area. On Day 14 after ovulation, US cineloops of ovaries were obtained in B mode and power flow to evaluate various morphological and vascular characteristics of the corpus luteum (CL), including luteal diameter, luteal area, and CL blood perfusion area. Cineloops were evaluated and analyzed using ultrasonic software, and in a computer with ImageJ software. To evaluate consistency in results between the two methods, there was evaluation utilizing paired t-test, Pearson correlation coefficient, Bland-Altman plot, and Linear Regression Test procedures to calculate proportion of bias between values for measurements of variables evaluated. Results indicated none of the values for variables before and after ovulation differed (P > 0.05). It, therefore, was concluded that utilization of ImageJ software is an efficacious biomedical technique to analyze ultrasonic images of morphological and vascular characteristics before and after ovulation in cattle.
AB - This study was conducted to compare the effectiveness of two methodologies in evaluating B- and Doppler-mode ultrasonic images: analysis using ultrasonic software and utilizing a computer with ImageJ software. To determine if ImageJ software utilization is an efficacious alternative to ultrasonic software device- analysis, there were comparisons of values when using the two methods for morphological and vascular characteristics of follicular dynamics and luteal function in 18 crossbred cattle. From day 8 of an ovarian dynamics synchronization treatment regimen period until the time of ovulation (Day 10), B-mode and power-flow ultrasonic cineloops were obtained every 12 h to assess follicular diameter, wall area, and wall blood perfusion area. On Day 14 after ovulation, US cineloops of ovaries were obtained in B mode and power flow to evaluate various morphological and vascular characteristics of the corpus luteum (CL), including luteal diameter, luteal area, and CL blood perfusion area. Cineloops were evaluated and analyzed using ultrasonic software, and in a computer with ImageJ software. To evaluate consistency in results between the two methods, there was evaluation utilizing paired t-test, Pearson correlation coefficient, Bland-Altman plot, and Linear Regression Test procedures to calculate proportion of bias between values for measurements of variables evaluated. Results indicated none of the values for variables before and after ovulation differed (P > 0.05). It, therefore, was concluded that utilization of ImageJ software is an efficacious biomedical technique to analyze ultrasonic images of morphological and vascular characteristics before and after ovulation in cattle.
KW - Bioimage
KW - Crossbred bovine
KW - Doppler
KW - Image processing
KW - Reproduction
UR - https://www.scopus.com/pages/publications/85121231580
U2 - 10.1016/j.anireprosci.2021.106907
DO - 10.1016/j.anireprosci.2021.106907
M3 - Artículo
C2 - 34923194
AN - SCOPUS:85121231580
SN - 0378-4320
VL - 236
JO - Animal Reproduction Science
JF - Animal Reproduction Science
M1 - 106907
ER -