Effect of salicylic acid and methyl jasmonate on conductivity and biomass growth in Phyllanthus acuminatus hairy roots

Hairy root cultures of Phyllanthus acuminatus Vahl represent a sustainable system for producing bioactive metabolites such as glycosides, lignans, and phenolics. This study evaluated the effect of methyl jasmonate (50.0 µM) and salicylic acid (50.0 and 200.0 µM) on biomass accumulation and culture medium conductivity in hairy roots of P. acuminatus. The highest dry weight (172.07 mg) and dry/fresh ratio (11.5%) were achieved with methyl jasmonate at 50.0 µM, indicating improved root morphology and growth efficiency. In contrast, salicylic acid at 200.0 µM significantly reduced biomass production. Conductivity values decreased throughout the culture period in all treatments, with a strong inverse correlation to biomass accumulation (R² > 80% in most cases). Regression models were established to describe fresh weight and conductivity over time. These findings suggest that methyl jasmonate at moderate concentrations enhances hairy root growth and provides a favorable kinetic profile for further extract production.