@inproceedings{6acaea595a354019835669675a1eda40,
title = "Deceived bilateral filter for improving the automatic cell segmentation and tracking in the NF-kB pathway without nuclear staining",
abstract = "In this work we implement an automatic videobased online cell tracking system for TNFα treated cells in the transcription factor NF-kB pathway, with the aim to provide p65 measurement approximation with time and individual cell resolution. The video used to test our proposed tracking system consists on cells transfected with fluorescent p65 protein. Such protein switches its location within the cell, among the nucleus and cytoplasm over time, causing important changes on its apparent morphology. This represents a major challenge for an automatic cell tracking system. Additionally, images in the tested microscopy footage present noise and contrast variations. For noise reduction, we modified the deceived bilateral filter (DBF), a denoising and edge sharpening non linear filter. The proposed modification of the DBF consists in an adaptive border enhancement. We tested the cell tracking algorithm with different preprocessing configurations achieving a maximum tracking accuracy improvement of 28\%, with the use of the modified DBF.",
keywords = "Automatic cell activity analysis, DBF, NF-kB pathway, Online tracking, Transcription factors",
author = "Ar{\'a}nzazu S{\'a}enz and Sa{\'u}l Calder{\'o}n and Jorge Castro and Rodrigo Mora and Francisco Siles",
note = "Publisher Copyright: {\textcopyright} Springer International Publishing Switzerland 2015; 6th Latin American Congress on Biomedical Engineering, CLAIB 2014 ; Conference date: 29-10-2014 Through 31-10-2014",
year = "2015",
doi = "10.1007/978-3-319-13117-7\_89",
language = "Ingl{\'e}s",
series = "IFMBE Proceedings",
publisher = "Springer Verlag",
pages = "345--348",
editor = "Ariel Braidot and Alejandro Hadad",
booktitle = "VI Latin American Congress on Biomedical Engineering, CLAIB 2014",
}