CRISPR/Cas9-Mediated Genome Editing in Indica Rice (Oryza sativa L. subsp. indica var. CR-5272)

Randall Rojas-Vásquez; Alejandro Hernández-Soto; Griselda Arrieta-Espinoza; Andrés Gatica-Arias

doi:10.1007/978-1-0716-3782-1_15

CRISPR/Cas9-Mediated Genome Editing in Indica Rice (Oryza sativa L. subsp. indica var. CR-5272)

Randall Rojas-Vásquez
, Alejandro Hernández-Soto
, Griselda Arrieta-Espinoza
, Andrés Gatica-Arias

University of Costa Rica
Vitroflora Labs

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

1 Scopus citations

Abstract

Tissue culture optimization protocols limit indica rice breeding. Such a challenge is vital because emergent techniques still rely on tissue culture methods and could allow the breeding of new varieties with higher production and toleration of adverse environmental effects caused by climate change. Genome editing technology, using CRISPR/Cas9, is a fast and precise method for accelerated plant breeding. It limited its use in indica subspecies because of the recalcitrant response to in vitro culture methods. This chapter describes a protocol for CRISPR/Cas9 editing in indica subspecies, specifically in the CR-5272 variety derived from parental lines IR-822, using Agrobacterium tumefaciens and biolistic transformation.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	257-271
Number of pages	15
DOIs	https://doi.org/10.1007/978-1-0716-3782-1_15
State	Published - 2024

Publication series

Name	Methods in Molecular Biology
Volume	2788
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 13 Climate Action

Keywords

Agrobacterium tumefaciens
Biolistics
CRISPR/Cas9
Embryogenic calli
In vitro regeneration

Access to Document

10.1007/978-1-0716-3782-1_15

Cite this

@inbook{4cf0ee46e1b64e8db3055dfe03c01e35,

title = "CRISPR/Cas9-Mediated Genome Editing in Indica Rice (Oryza sativa L. subsp. indica var. CR-5272)",

abstract = "Tissue culture optimization protocols limit indica rice breeding. Such a challenge is vital because emergent techniques still rely on tissue culture methods and could allow the breeding of new varieties with higher production and toleration of adverse environmental effects caused by climate change. Genome editing technology, using CRISPR/Cas9, is a fast and precise method for accelerated plant breeding. It limited its use in indica subspecies because of the recalcitrant response to in vitro culture methods. This chapter describes a protocol for CRISPR/Cas9 editing in indica subspecies, specifically in the CR-5272 variety derived from parental lines IR-822, using Agrobacterium tumefaciens and biolistic transformation.",

keywords = "Agrobacterium tumefaciens, Biolistics, CRISPR/Cas9, Embryogenic calli, In vitro regeneration",

author = "Randall Rojas-V{\'a}squez and Alejandro Hern{\'a}ndez-Soto and Griselda Arrieta-Espinoza and Andr{\'e}s Gatica-Arias",

note = "Publisher Copyright: {\textcopyright} The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.",

year = "2024",

doi = "10.1007/978-1-0716-3782-1\_15",

language = "Ingl{\'e}s",

series = "Methods in Molecular Biology",

publisher = "Humana Press Inc.",

pages = "257--271",

booktitle = "Methods in Molecular Biology",

}

CRISPR/Cas9-Mediated Genome Editing in Indica Rice (Oryza sativa L. subsp. indica var. CR-5272). / Rojas-Vásquez, Randall; Hernández-Soto, Alejandro; Arrieta-Espinoza, Griselda et al.
Methods in Molecular Biology. Humana Press Inc., 2024. p. 257-271 (Methods in Molecular Biology; Vol. 2788).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

TY - CHAP

T1 - CRISPR/Cas9-Mediated Genome Editing in Indica Rice (Oryza sativa L. subsp. indica var. CR-5272)

AU - Rojas-Vásquez, Randall

AU - Hernández-Soto, Alejandro

AU - Arrieta-Espinoza, Griselda

AU - Gatica-Arias, Andrés

N1 - Publisher Copyright: © The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.

PY - 2024

Y1 - 2024

N2 - Tissue culture optimization protocols limit indica rice breeding. Such a challenge is vital because emergent techniques still rely on tissue culture methods and could allow the breeding of new varieties with higher production and toleration of adverse environmental effects caused by climate change. Genome editing technology, using CRISPR/Cas9, is a fast and precise method for accelerated plant breeding. It limited its use in indica subspecies because of the recalcitrant response to in vitro culture methods. This chapter describes a protocol for CRISPR/Cas9 editing in indica subspecies, specifically in the CR-5272 variety derived from parental lines IR-822, using Agrobacterium tumefaciens and biolistic transformation.

AB - Tissue culture optimization protocols limit indica rice breeding. Such a challenge is vital because emergent techniques still rely on tissue culture methods and could allow the breeding of new varieties with higher production and toleration of adverse environmental effects caused by climate change. Genome editing technology, using CRISPR/Cas9, is a fast and precise method for accelerated plant breeding. It limited its use in indica subspecies because of the recalcitrant response to in vitro culture methods. This chapter describes a protocol for CRISPR/Cas9 editing in indica subspecies, specifically in the CR-5272 variety derived from parental lines IR-822, using Agrobacterium tumefaciens and biolistic transformation.

KW - Agrobacterium tumefaciens

KW - Biolistics

KW - CRISPR/Cas9

KW - Embryogenic calli

KW - In vitro regeneration

UR - https://www.scopus.com/pages/publications/85191377059

U2 - 10.1007/978-1-0716-3782-1_15

DO - 10.1007/978-1-0716-3782-1_15

M3 - Capítulo

C2 - 38656519

AN - SCOPUS:85191377059

T3 - Methods in Molecular Biology

SP - 257

EP - 271

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

CRISPR/Cas9-Mediated Genome Editing in Indica Rice (Oryza sativa L. subsp. indica var. CR-5272)

Abstract

Publication series

UN SDGs

Keywords

Access to Document

Other files and links

Fingerprint

Cite this