TY - JOUR
T1 - Ameliorative Effects of Daphnopsis costaricensis Extract against Oxazolone-Induced Atopic Dermatitis-like Lesions in BALB/c Mice
AU - Bae, Yunji
AU - Kim, Taeyoung
AU - Park, Nojune
AU - Choi, Sangho
AU - Yi, Dongkeun
AU - Soto, Silvia
AU - Zamora, Nelson
AU - Kim, Sunam
AU - Yang, Minhye
PY - 2022/11
Y1 - 2022/11
N2 - The genus Daphnopsis has been traditionally used as a purgative, diuretic, stimulant, and psoriasis treatment. In this study, the anti-AD (atopic dermatitis) activities of the Daphnopsis costaricensis EtOH extract (DCE) were investigated in an oxazolone (OX)-induced mouse model of AD, and the anti-inflammatory effects of its active compounds were confirmed in PI-sensitized or IgE/DNP-BSA-sensitized RBL-2H3 cells. DCE improved the symptoms of OX-induced inflammatory dermatitis (swelling, erythema, and increased ear thickening) in OX-induced BALB/c mice ears and reduced epidermal thickness and mast cell infiltration. Eleven flavonoid compounds were isolated from DCE, and two compounds (7,8-dimethoxyflavone and 7,2’-dimethoxyflavone) significantly inhibited IL-4 overexpression in PI-induced RBL-2H3 cells and mast cell degranulation in IgE + DNP-BSA-induced RBL-2H3 cells. Our study indicates that DCE and two compounds (7,8-dimethoxyflavone and 7,2’-dimethoxyflavone) might effectively improve inflammatory and atopic skin symptoms.
AB - The genus Daphnopsis has been traditionally used as a purgative, diuretic, stimulant, and psoriasis treatment. In this study, the anti-AD (atopic dermatitis) activities of the Daphnopsis costaricensis EtOH extract (DCE) were investigated in an oxazolone (OX)-induced mouse model of AD, and the anti-inflammatory effects of its active compounds were confirmed in PI-sensitized or IgE/DNP-BSA-sensitized RBL-2H3 cells. DCE improved the symptoms of OX-induced inflammatory dermatitis (swelling, erythema, and increased ear thickening) in OX-induced BALB/c mice ears and reduced epidermal thickness and mast cell infiltration. Eleven flavonoid compounds were isolated from DCE, and two compounds (7,8-dimethoxyflavone and 7,2’-dimethoxyflavone) significantly inhibited IL-4 overexpression in PI-induced RBL-2H3 cells and mast cell degranulation in IgE + DNP-BSA-induced RBL-2H3 cells. Our study indicates that DCE and two compounds (7,8-dimethoxyflavone and 7,2’-dimethoxyflavone) might effectively improve inflammatory and atopic skin symptoms.
KW - 7,2 '-dimethoxyflavone
KW - 7,8-dimethoxyflavone
KW - Daphnopsis costariensis
KW - Anti-inflammatory
KW - Atopic dermatitis
KW - Beta-hexosaminidase
KW - Interleukin 4
KW - Daphnopsis costariensis;
KW - atopic dermatitis
KW - Anti-inflammatory
KW - 7,8-dimethoxyflavone
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=tec-sibitec&SrcAuth=WosAPI&KeyUT=WOS:000881471100001&DestLinkType=FullRecord&DestApp=WOS_CPL
U2 - 10.3390/nu14214521
DO - 10.3390/nu14214521
M3 - Artículo
C2 - 36364783
VL - 14
JO - Nutrients
JF - Nutrients
IS - 21
M1 - 4521
ER -