Papp Fase I: Establecimiento de un modelo intestinal in vitro para el cálculo del coeficiente de permeabilidad aparente de compuestos bioactivos

Intestinal permeability is one of the main factors affecting the absorption of orally administered
substances. In vitro permeability models can help to identify substances whose pharmacokinetics
are deficient from early stages of investigation and, at the same time, facilitate the prediction of
permeability of promising substances, to continue with later clinical phases [1]. Multiple
compounds, mainly of plant origin, with high biomedical potential have been studied at the
Biotechnology Research Center (CIB) of the School of Biology, but their absorption profile is
unknown; thus, appropriate methods to assess intestinal permeability are necessary. The
differentiated human Caco-2 cells are a standard in vitro model of human intestinal permeability to
determine the oral bioavailability of substances [2, 3]. Therefore, the objective of this research
proposal is to establish at CIB the three compartment system (lumen / apical, intracellular, and
basolateral) with differentiated human Caco-2 cells for the calculation of the apparent permeability
coefficient of bioactive compounds, and its consequent use for the prediction of bioavailability in
animals models and in later clinical phases

Establecer el sistema intestinal in vitro de tres compartimientos con células
humanas Caco-2 diferenciadas para el cálculo del coeficiente de permeabilidad aparente.

Biotecnología Médica