The evaluation of physiological parameters and the proteomic profile of sperm could enhance the predictive
capacity of fertility of reproducers/ejaculates. The study of both physiological (motility, viability or polarization
of the mitochondrial membrane) and molecular (protein and metabolic profile) parameters, allowing understand
what is happening during sperm conservation. Also, the knowledge generated through the study will optimize
the conservation of sperm cells and maintain their fertilizing capacity for a longer time. This allows a
considerable improvement for reproductive performance, selection intensity, sustainability and economic
viability in these agricultural systems. Therefore, the aim of this project is the study of the parameters related
to energy metabolism and the protein profile of spermatozoa in fresh, refrigerated and cryopreserved semen,
in livestock species.