Evaluación de un sistema biológico eucariota (Nicotiana tabacum) para la producción de insulina humana recombinante, con miras al escalamiento en biorreactor

Insulin is a protein of great importance for the good metabolic development of the human being, since it transports glucose to the pancreas to be metabolized. However, due to genetic and nutritional problems, there is a high number of people who do not produce this protein, which is why they develop the disease called diabetes, which is a high cause of patients and deaths worldwide. In order to generate efficient medicines and therapies, it is necessary to establish expression platforms to produce this protein in an efficient, scalable and safe way. Nicotiana tabacum is a eukaryotic organism that presents an optimal metabolism for the expression of recombinant proteins. This is due to its ability to make post-transductional modifications, scalability and available techniques for its genetic transformation. The present study seeks to generate transformed tobacco cells using this species from two different approaches (plastid and nuclear transformation) that can express, scale and purify proinsulin to evaluate its biological activity in human cells.

Evaluar un sistema de producción escalable de la proteína recombinante
humana insulina en suspensiones celulares de tabaco (Nicotiana tabacum).

Área de investigación de la Escuela de Biología: Biotecnología Vegetal y Aplicaciones Biomédicas