Establecimiento de una estrategia de cultivo invitro de Cannabis sativa en Saccharomyces cereviceae para la producción de fibra y cannabinoides (CBD, CBG)

Hemp (Cannabis sativa) has been consumed for medicinal, spiritual, cultural and recreational
purposes; and is characterized by its ability to synthesize more than 90 different types of
phytocannabinoids. The cultivation of hemp for cannabinoid extraction presents several challenges,
mainly due to its genetic and phytochemical diversity. In vitro tissue culture techniques using
different explants and growth regulators have been performed, however, there are still several
challenges to overcome. The development of genetic engineering can improve the yield of
biosynthesis of different cannabinoids by using microorganisms for heterologous production.
The Costa Rican Congress approved, in a second and final vote, the legalization of medical
cannabis and industrial hemp, which is why the country will require technology and material related
to the cultivation of hemp in the country.
The present proposal intends, on the one hand, to establish a model of in vitro cultivation and in
acclimatized chambers of different varieties of hemp, to obtain varieties adapted to different climates
and conditions. With the results obtained, it will be possible to generate inputs for producers to
generate higher yields in hemp production.
On the other hand, a synthetic system to produce cannabinoids in washes will be developed, to
obtain pure compounds such as CBD and CBG, avoiding contamination problems with other
compounds of little commercial interest such as THC. With these results, high value compounds
could be generated in a more efficient, pure and economical way.

Establecer una estrategia in vitro de cultivos de Cannabis sativa y estudios de
regeneración para optimizar la producción de fibra y cannabinoides (CBD, CBG)

Biotecnologia vegetal