Desarrollo de patrón de estimulación mecánica para inducir la diferenciación de mioblastos a músculo esquelético

The tissues of the human body experience different types of stimuli, including mechanical
stimulation. This stimulation greatly affects cell’s vital processes such as: embryonic
development, growth, differentiation and even cell death. Studying the specific conditions
on how skeletal muscular tissue develops from progenitor cells is a crucial problem to solve.
This will provide insight into obtaining in-vitro three-dimensional tissues that can be used
as study models study models. The main objective of this project is to investigate a
mechanical stimulation pattern that allows differentiation of C2C12 cells inoculated in threedimensional scaffolds into mature muscular cells in order to achieve an in-vitro muscle
tissue that may be used for further studies.
The cell growth conditions that allow the greatest viability were standardized previously
inside a bioreactor developed in a previous research. Microfilaments are fabricated using
PCL, these are sterilized and coated with type I collagen to enhance biocompatibility. The
scaffold is inoculated with the C2C12 cells. The scaffold will be placed in the mechanical
stimulation bioreactor with different types mechanical stimulation to determine the optimal
conditions of cell growth and thus achieve the best proliferation, morphology and
differentiation into mature and functional muscle fibers. Once this objective has been
achieved, it is intended to optimize this study model to apply it in research related to tissue
regeneration, the effect of drugs, among others.

Desarrollar un patrón de estimulación mecánica para inducir la diferenciación de
mioblastos sembrados en un andamio polimérico tridimensional que simule la
fisiología normal de un músculo esquelético

Nuevos materiales y Energías renovables, Escuela de Ciencia e Ingeniería de los
Materiales
Biotecnología médica, Escuela de Biología
Biofísica, Escuela de Física