Project Details
Description
Xylitol is a sugar polyol that has been used in the food industry as a low calorie sweetener used in
products such as toothpaste, chewing gum, mouthwash and nasal spray. The rice husk is a waste
that contains cellulose, which can be metabolized in xylose and glucose, and the xylose in turn can
be metabolized in xylitol by the use of microorganisms, reingeneered to be able to process this
waste and use it as a substrate, being this a safer and more environmentally friendly option, which
is why this proposal suggests generating a bacterial platform using the Pseudomonas putida
bacterium, and being able to take advantage of rice husk as a carbon source to generate xylitol. P.
putida is a saprophyte soil bacterium, metabolically versatile, fast growing and easy to handle in the
laboratory, with the ability to use organic waste in aerobic environments, thus playing a key role in
maintaining environmental quality. No strains of P. putida are known to be pathogens of plants or
animals, and this saprophyte species is considered harmless to the environment. In addition, in the
laboratory of Synthetic Biology of the Center for Research in Biotechnology of the Technological of
Costa Rica, already has a strain of P. putida modified so that it does not produce flagella, which,
after carrying out the bioinformatic studies and once synthesized the genetic pieces will be
transformed into a laboratory by electroporation, the transformation will be verified by molecular
methods such as PCR, in addition, the production of Xylitol will be verified by means of HPLC. Thus,
the objective of this research is to design a microbial platform capable of generating xylitol from rice
husks using synthetic biology.
products such as toothpaste, chewing gum, mouthwash and nasal spray. The rice husk is a waste
that contains cellulose, which can be metabolized in xylose and glucose, and the xylose in turn can
be metabolized in xylitol by the use of microorganisms, reingeneered to be able to process this
waste and use it as a substrate, being this a safer and more environmentally friendly option, which
is why this proposal suggests generating a bacterial platform using the Pseudomonas putida
bacterium, and being able to take advantage of rice husk as a carbon source to generate xylitol. P.
putida is a saprophyte soil bacterium, metabolically versatile, fast growing and easy to handle in the
laboratory, with the ability to use organic waste in aerobic environments, thus playing a key role in
maintaining environmental quality. No strains of P. putida are known to be pathogens of plants or
animals, and this saprophyte species is considered harmless to the environment. In addition, in the
laboratory of Synthetic Biology of the Center for Research in Biotechnology of the Technological of
Costa Rica, already has a strain of P. putida modified so that it does not produce flagella, which,
after carrying out the bioinformatic studies and once synthesized the genetic pieces will be
transformed into a laboratory by electroporation, the transformation will be verified by molecular
methods such as PCR, in addition, the production of Xylitol will be verified by means of HPLC. Thus,
the objective of this research is to design a microbial platform capable of generating xylitol from rice
husks using synthetic biology.
General Objective
Rediseñar genéticamente la bacteria Pseudomonas putida, mediante biología
sintética de modo que sea capaz de generar xilitol utilizando cascarilla de arroz como fuente de
carbono
sintética de modo que sea capaz de generar xilitol utilizando cascarilla de arroz como fuente de
carbono
Research Lines
Área de la Escuela de Biología: Biotecnología Ambiental.
| Status | Finished |
|---|---|
| Effective start/end date | 1/01/23 → 31/12/24 |
Keywords
- Pseudomonas putida
- agroindustrial waste
- xylanases
- cellulases
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